Analysis and Development of Targeting Strategies for Bacterial Sugars

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Erickson, K. (2018). Analysis and Development of Targeting Strategies for Bacterial Sugars. Unc Charlotte Electronic Theses And Dissertations.
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Abstract

Bacterial cell surface exopolysaccharides aid in protecting microbes from the defense mechanisms of the host, and differ substantially in structure between species. Exploiting these structural differences would be advantageous to target certain types of bacteria over others for organism selective antimicrobial agents or reagents to detect specific bacteria in the environment. Using the N-linked oligosaccharide from Campylobacter jejuni as a model system, I have developed a method for producing the oligosaccharide repeat unit of a bacterial surface polysaccharide. Using this system, I will now develop a new way to immobilize bacterial sugars for the isolation of agents that specifically interact with the cell surface of bacteria. Not only was this completed for the glycan from C. jejuni, but also for the newly elucidated colanic acid tetrasaccharide from E. coli. Alongside the developed immobilization platform, our lab has acquired mutant strains of E. coli, one that contains the N-linked oligosaccharide, and one that does not. Utilizing the difference between the E. coli strains, I have selected for agents specific to the C. jejuni polymer in a whole cell experiment. The selection of the whole cell and immobilized agents have been completed for the two glycans of interest and for two strains of E. coli and visualized by gel electrophoresis, flow cytometry and plate reader analysis. For the immobilization method, synthesis of an analogue with a different chemical handle has been completed, followed by successful building of two different sugar repeating units onto the new analogue, as seen by HPLC analysis and ESI-MS analysis.

Details
Author

Erickson, Katelyn

Title

Analysis and Development of Targeting Strategies for Bacterial Sugars

Physical Description

1 online resource (152 pages) : PDF

Date

2018

Degree Granting Institution

University of North Carolina at Charlotte

Abstract

Bacterial cell surface exopolysaccharides aid in protecting microbes from the defense mechanisms of the host, and differ substantially in structure between species. Exploiting these structural differences would be advantageous to target certain types of bacteria over others for organism selective antimicrobial agents or reagents to detect specific bacteria in the environment. Using the N-linked oligosaccharide from Campylobacter jejuni as a model system, I have developed a method for producing the oligosaccharide repeat unit of a bacterial surface polysaccharide. Using this system, I will now develop a new way to immobilize bacterial sugars for the isolation of agents that specifically interact with the cell surface of bacteria. Not only was this completed for the glycan from C. jejuni, but also for the newly elucidated colanic acid tetrasaccharide from E. coli. Alongside the developed immobilization platform, our lab has acquired mutant strains of E. coli, one that contains the N-linked oligosaccharide, and one that does not. Utilizing the difference between the E. coli strains, I have selected for agents specific to the C. jejuni polymer in a whole cell experiment. The selection of the whole cell and immobilized agents have been completed for the two glycans of interest and for two strains of E. coli and visualized by gel electrophoresis, flow cytometry and plate reader analysis. For the immobilization method, synthesis of an analogue with a different chemical handle has been completed, followed by successful building of two different sugar repeating units onto the new analogue, as seen by HPLC analysis and ESI-MS analysis.

Genre

doctoral dissertations

Subjects--Topics

Chemistry
Biochemistry

Degree

Ph.D.

Keywords

Aptamer
Bacteria
Sugar

Subject Area

Chemistry

Advisor(s)

Troutman, Jerry

Committee Members

Afonin, Kirill
Vivero-Escoto, Juan
Richardson, Christine
Ogle, Craig

Degree Note

Thesis (Ph.D.)--University of North Carolina at Charlotte, 2018.

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Rights Holder Information

Copyright is held by the author unless otherwise indicated.

Identifier

Erickson_uncc_0694D_11893

Permalink

http://hdl.handle.net/20.500.13093/etd:1713