Production of Immunomodulatory Proteins in Glycine max for use in Diagnostics and as Therapeutics

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Powell, R. (2010). Production of Immunomodulatory Proteins in Glycine max for use in Diagnostics and as Therapeutics. Unc Charlotte Electronic Theses And Dissertations.
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Abstract

ABSTRACTREBECCA DIANE POWELL. Production of Immunomodulatory Proteins in Glycine max for Use in Diagnostics and as Therapeutics. (Under the direction of DR. KENNETH L. BOST) Immunomodulatory proteins can be utilized in diagnostic assays, as tolerance therapies and in vaccine development. In the following studies, we describe the expression, characterization and use of two such proteins from the soybean expression platform: human thyroglobulin (hTG) and merozoite surface protein 119 (MSP119). We characterize the 1% TSP expression of human thyroglobulin, the primary auto antigen in Hashimoto's thyroid disease, in soybean. Our laboratory successfully purifies this vital diagnostic analyte and indicates its biological equivalency to human-derived thyroglobulin. Furthermore, we demonstrate the usefulness of soy-derived hTG in preventing and inhibiting the progression of Hashimoto's thyroid disease in the murine model. While unable to express MSP 119 at the same level in the soybean seed, we use the following studies to further characterize the expression constructs that generate the greatest success in producing a soy-derived protein and optimize the process that leads to the use of oral delivery in vaccine development.

Details
Author

Powell, Rebecca

Title

Production of Immunomodulatory Proteins in Glycine max for use in Diagnostics and as Therapeutics

Physical Description

1 online resource (106 pages) : PDF

Date

2010

Degree Granting Institution

University of North Carolina at Charlotte

Abstract

ABSTRACTREBECCA DIANE POWELL. Production of Immunomodulatory Proteins in Glycine max for Use in Diagnostics and as Therapeutics. (Under the direction of DR. KENNETH L. BOST) Immunomodulatory proteins can be utilized in diagnostic assays, as tolerance therapies and in vaccine development. In the following studies, we describe the expression, characterization and use of two such proteins from the soybean expression platform: human thyroglobulin (hTG) and merozoite surface protein 119 (MSP119). We characterize the 1% TSP expression of human thyroglobulin, the primary auto antigen in Hashimoto's thyroid disease, in soybean. Our laboratory successfully purifies this vital diagnostic analyte and indicates its biological equivalency to human-derived thyroglobulin. Furthermore, we demonstrate the usefulness of soy-derived hTG in preventing and inhibiting the progression of Hashimoto's thyroid disease in the murine model. While unable to express MSP 119 at the same level in the soybean seed, we use the following studies to further characterize the expression constructs that generate the greatest success in producing a soy-derived protein and optimize the process that leads to the use of oral delivery in vaccine development.

Genre

doctoral dissertations

Subjects--Topics

Molecular biology

Degree

Ph.D.

Keywords

Malaria
Oral Tolerance
Soybean
Thyroiditis
Vaccines

Subject Area

Biology

Advisor(s)

Bost, Kenneth
Piller, Kenneth

Committee Members

Schrum, Laura
Marriott, Ian
Lee, Charles

Degree Note

Thesis (Ph.D.)--University of North Carolina at Charlotte, 2010.

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Rights Holder Information

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Identifier

Powell_uncc_0694D_10141

Permalink

http://hdl.handle.net/20.500.13093/etd:572