Intracellular Subsurface Imaging Using a Hybrid Shear-Force Feedback/ Scanning Quantitative Phase Microscopy Technique
1 online resource (165 pages) : PDF
University of North Carolina at Charlotte
Quantitative phase microscopy (QPM) allows for the imaging of translucent or transparent biological specimens without the need for exogenous contrast agents. This technique is usually applied towards the investigation of simple cells such as red blood cells which are typically enucleated and can be considered to be homogenous. However, most biological cells are nucleated and contain other interesting intracellular organelles. It has been established that the physical characteristics of certain subsurface structures such as the shape and roughness of the nucleus is well correlated with onset and progress of pathological conditions such as cancer. Although the acquired quantitative phase information of biological cells contains surface information as well as coupled subsurface information, the latter has been ignored up until now. A novel scanning quantitative phase imaging system unencumbered by 2π ambiguities is hereby presented. This system is incorporated into a shear-force feedback scheme which allows for simultaneous phase and topography determination. It will be shown how subsequent image processing of these two data sets allows for the extraction of the subsurface component in the phase data and in-vivo cell refractometry studies. Both fabricated samples and biological cells ranging from rat fibroblast cells to malaria infected human erythrocytes were investigated as part of this research. The results correlate quite well with that obtained via other microscopy techniques.
BIO-IMAGINGCELLULAR IMAGINGNSOMOPTICAL IMAGINGQUANTITATIVE PHASE IMAGINGSUBSURFACE IMAGING
Optical Science and Engineering
Farahi, FaramarzGbur, GregHocken, RobertSteck, Todd
Thesis (Ph.D.)--University of North Carolina at Charlotte, 2009.
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