Next-Generation Sequencing Based Quantification of Microbial Communities and Gene Pathways

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Jones, R. (2016). Next-Generation Sequencing Based Quantification of Microbial Communities and Gene Pathways. Unc Charlotte Electronic Theses And Dissertations.
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Abstract

Humans act as a host to trillions of microorganisms. The collective of these microorganisms is called a host’s microbiota. There is a growing interest in the bacterial composition of the gut microbiome of humans (structure) and the role of the microbiome in human diseases (function). Many methods are used to define the structure and the function of a microbial community and there are concerns about how the use of these varying methods can impact the reproducibility of microbiome research. In this dissertation, we aimed to determine how different measurement techniques impacts the understanding of the structure and function of the gut bacterial communities in humans and in model systems such as non-human primates and rodents. Along with determining these different techniques to quantify the structure and function of the gut microbiome, this dissertation applies these different techniques to determine how dietary sugars impact microbial community composition and determine factors that are associated with the gut microbiome in patients with colorectal adenomas, a benign tumor which is often times a precursor to colorectal cancer.

Details
Author

Jones, Roshonda

Title

Next-Generation Sequencing Based Quantification of Microbial Communities and Gene Pathways

Physical Description

1 online resource (138 pages) : PDF

Date

2016

Degree Granting Institution

University of North Carolina at Charlotte

Abstract

Humans act as a host to trillions of microorganisms. The collective of these microorganisms is called a host’s microbiota. There is a growing interest in the bacterial composition of the gut microbiome of humans (structure) and the role of the microbiome in human diseases (function). Many methods are used to define the structure and the function of a microbial community and there are concerns about how the use of these varying methods can impact the reproducibility of microbiome research. In this dissertation, we aimed to determine how different measurement techniques impacts the understanding of the structure and function of the gut bacterial communities in humans and in model systems such as non-human primates and rodents. Along with determining these different techniques to quantify the structure and function of the gut microbiome, this dissertation applies these different techniques to determine how dietary sugars impact microbial community composition and determine factors that are associated with the gut microbiome in patients with colorectal adenomas, a benign tumor which is often times a precursor to colorectal cancer.

Genre

doctoral dissertations

Subjects--Topics

Bioinformatics

Degree

Ph.D.

Keywords

16S RRNA
Metagenomics
Whole-Genome Shotgun Sequencing

Subject Area

Bioinformatics

Advisor(s)

Fodor, Anthony

Committee Members

Sha, Wei
Su, Zhengchang
Shi, Xinghua
Richardson, Christine

Degree Note

Thesis (Ph.D.)--University of North Carolina at Charlotte, 2016.

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Rights Holder Information

Copyright is held by the author unless otherwise indicated.

Identifier

Jones_uncc_0694D_11243

Permalink

http://hdl.handle.net/20.500.13093/etd:1148