Cholera Toxin Subunit B-Mediated Intracellular Trafficking of Mesoporous Silica Nanoparticles Toward the Endoplasmic Reticulum
1 online resource (57 pages) : PDF
University of North Carolina at Charlotte
In recent decades, pharmaceutical research has led to the development of numerous treatments for human disease. Nanoscale delivery systems have the potential to maximize therapeutic outcomes by enabling target specific delivery of these therapeutics. The intracellular localization of many of these materials however, is poorly controlled, leading to sequestration in degradative cellular pathways and limiting the efficacy of their payloads. Numerous proteins, particularly bacterial toxins, have evolved mechanisms to subvert the degradative mechanisms of the cell. Here, we have investigated a possible strategy for shunting intracellular delivery of encapsulated cargoes from these pathways by modifying mesoporous silica nanoparticles (MSNs) with the well-characterized bacterial toxin Cholera toxin subunit B (CTxB). Using established optical imaging methods we investigated the internalization, trafficking, and subcellular localization of our modified MSNs in an in vitro animal cell model. We then attempted to demonstrate the practical utility of this approach by using CTxB-modified mesoporous silica nanoparticles to deliver propidium iodide, a membrane-impermeant fluorophore.
CHOLERA TOXINMESOPOROUS SILICANANOMEDICINE
Dréau, DidierTroutman, JerryKrueger, Joanna
Thesis (M.S.)--University of North Carolina at Charlotte, 2015.
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